Microscope Systems Limited, Microscopes UK > pE-4000 > UK Microscope Supplier: Olympus - Yenway - Motic - CoolLED - Micropix
Olympus Microscopy > pE-4000 > Yenway Microscopes > pE-4000 > Motic Microscopes > pE-4000 > CoolLED > pE-4000 > Micropix Microscope Cameras > pE-4000 >
Tel: 01360 660 572 | Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Microscope Systems Limited, Microscopes UK > pE-4000 >

Tel: 01360 660 572
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

THE UNIVERSAL LIGHT SOURCE                            > pE-4000 >



The pE-4000 sets the standard as the universal light source for fluorescence microscopy.  Users can operate the system as a simple white light source (replacing a conventional mercury-based light source), or as an advanced, fully-controllable, excitation and stimulation source.  The flexibility and extensive functionality of the pE-4000 broadens the range of illumination options in core facilities.




At the centre of the pE-4000 is CoolLED's novel, patent pending, wavelength-grouping concept which offers more power in an efficient system design.  Wavelength-grouping ensures optimal compatibility with all single and multi-band filter sets.






16 Selectable wavelengths - No Modularity, Compatible with all filter sets

 > pE-4000 >

Broadest Spectrum and Brightest LEDs

 > pE-4000 >




  • Simple on/off
  • Precise intensity control
  • Easy to use, no training required
  • Pre-sets allowing lab manager to match white spectrum to exiting filter cubes
  • Higher contrast images from matched-white spectrum


 > pE-4000 >




  • Individual LED wavelength selection
  • Rapid switching between LED wavelengths enables capture of high speed events
  • TTL & USB interfaces with imaging packages
  • Excitation filters can be fitted in optical path for controlled switching with no moving parts
  • Analogue for dynamic intensity control
  • Optical feedback for applications requiring higher stability
  • Internal function generator for electrophysiology and optogenetics applications
  • Compatible with all single and multi-band filter sets


The pE-4000 system:

  • Excellent field uniformity at sample
  • No mercury
  • Long Life:  25,000 hours
  • No bulb changing, bulb alignment or warm up process
  • Quiet operation
  • High efficiency
  • Wide range of microscope adaptors

pE-4000 Technical Information


pE-4000 System Main unit with complete set of wavelengths, dual function manual control pod, power supply block, mains cable, USB cable
Light delivery Single liquid light guide or fiber options
Collimating Optics Range of collimating optics and adaptors to fit most fluorescence microscopes

LED Wavelengths are divided across 4 channels with each channel having individual control. 

Wavelength Power  FWHM  Wavelength Power  FWHM  Wavelength Power  FWHM  Wavelength Power  FWHM 
365 15.12 12 460 240.19 19 525 39.00 34 635 123.88 16
385 59.39 11 470 192.29 20 550 166.74 85 660 159.01 21
405 188.32 18 490 56.00 32 580 166.74 85 740 36.62 29
435 138.75 16 500 59.26 29 595 32.52 15 770 15.61 28

Powers measured at sample plane of research grade microscope using 100% mirror in cube and 10X objectives. (Figures are typical values) Note wavelength settings 550nm and 580nm use single broad, high intensity peak (see spectrum pE-4000 page)

Control & Interface

Manual Dual function manual control pod for White mode and Advanced mode
Remote Via USB for independent on/off and intensity control of each channel.  Triggering speed
  Via 4 TTL iputs for independent on/off control of each channel.  Triggering speed
  Via single TTL for on/off control of manual or software selected channels
  Via 4 analogue inputs 0-10V, 0-300Hz for dynamic control of intensity from external analogue signals
Synch Out 4 TTL outputs for each channel - active high
  1 TTL output for any channel - active high
Programmable Interface 4 TTL outputs for on/off control of peripherals (transmitted light sources, stages etc)
  4 analogue outputs for intensity control of peripherals (can be programmed to mirror LED intensities for channel control) 0-10V full scale.
Function Generator Internally generated sine, pulse and ramps for each channel programmed via pod
Connectivity USB (B type) for PC connection.  All other TTL and Analogue inputs/outputs via 25way 'D-type' female connector (optional rear mounting expansion box available for BNC connectivity).
Imaging Software Recognised as 'CoolLED pE-2 peripheral' under common software e.g. MicroManager, MetaMorph, cellSens, NIS Elements, ImagePro etc.


Power requirements 110-240Va.c. 50/60Hz, 2.5A   
Power Consumption Standby (i.e. no LEDs on) Max 7W
  Single wavelength operation Max 41W
  Dual wavelength operation Max 75W
  Triple wavelength operation Max 93W
  Quad wavelength operation Max 112W


Main Unit 150mm(w) x 220mm(d) x 260mm(h) - Weight 3.5kg
Control Pod 154mm(w) x 135mm(d) x 40mm(h) - Weight 0.95kg
Power Supply 164mm(w) x 64mm(d) x 35mm(h) - Weight 0.58kg

Order Codes

pE-4000-L-SYS-ZZ Main unit, control pod, power supply plus cables for use with 3mm liquid light guide
pE-4000-F-SYS-ZZ Main unit, control pod, power supply plus cables for use with SMA terminated fiber
pE-1904 3mm diameter, 1m long liquid light guide
pE-1908 3mm diameter, 3m long liquid light guide
pE-10400-YYY Microscope adaptor with collimating optics.  To specify microscope code (YYY) see
Fiber Options see for further information
pE-4000-EB25D Rear mounting expansion box for 25way D-type to BNC connectivity
Power Cable To specify local power cable (ZZ). 10 = Australia, 20 = Europe, 30 = UK, 40 = USA
Warranty System:  12 months, LEDs:  25,000 hours


Contact MSL

For detailed quotations, and to discuss all your possible microscopy requirements,

Contact us,
Microscope Systems Limited:
T: 01360 660 572
M: 07973 971 143
E: This email address is being protected from spambots. You need JavaScript enabled to view it.


I want to thank you very much for working so well for us today – I’ve strongly recommended that you be recalled next year.

- Lesley McEvoy
North Atlantic Fisheries College, Shetland

Well executed service and repair of Olympus & Prior microscopes, one happy customer. James provided an excellent service

- Bill Revie
Construction Materials Consultancy

Contact Us

Tel: 01360 660 572
Mob: 07973 971 143
Fax:  01360 660 572

Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
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