Optically ahead of the curve - the UIS2 optical system
At the heart of any light microscope is the optical system; a series of lenses, prisms and filters designed to magnify the target area of a sample whilst resolving more detail from it. This is far more complex than this basic overview portrays: Since as well as enabling samples to be imaged they allow the introduction and control of light and the projection of images to the eyes (or to a camera). On top of all of this are a range of optical aberrations that can affect microscopy if not properly corrected for. The Olympus UIS2 optical components have been developed to provide the perfect optical system; setting a new standard in precision and clarity.
Dedicated to Fluorescence
As well as the objectives, the new UIS2 fluorescent mirror cube range and 8 position mirror turret have been optimised for flexible and high quality imaging. An ultra high quality coating process provides excellent transmission and exceptionally steep cut-off slopes and the low reflection interior surfaces eliminate over 99% of stray light. As a result, these filter cubes ensure the highest sensitivity and best colour separation, which coupled with the low auto-fluorescent glass materials, further improves the S:N ratio. In addition the mirror cubes can be exchanged without the need for any tools, making updates very quick and easy.
A novel fluorescence illumination concept
Fluorescence illumination has been at the forefront of life science microscopy for many years. With the BX3 systems, Olympus has incorporated a number of design features and components that will enable fluorescence to maintain this leading position. Of these advances the most important is the introduction of a novel fly-eye lens system which ensures that the field of view is illuminated homogeneously. As well as providing such flawless illumination, the fly-eye system also provides simpler burner alignment.
The Olympus BX53 microscope is designed to be ambidextrous so you don’t have to be! For example, the light control dial is placed in the centre of the frame, and the transmitted attenuation filters and fluorescence shutters can be operated from either side, as can the focus knobs. If the microscope is fitted with an Olympus digital camera, a remote exposure button is available, which can also be placed on either side of the frame, enabling uninterrupted image framing and capture. As a result you maintain full control over you workspace, consequently improving workflow efficiency as everything is exactly where you want it.
Motorisation – Pushing the boundaries
The Olympus BX3 microscopes offer even greater application flexibility via a series of motorisation and automation options, all of which can be controlled along with illumination and image capture via the cellSens software. The addition of these motorised and automated components therefore provides excellent potential for enhanced experimentation. The automation options work well in union with each other, but can be implemented as independent solutions, so the user only has to motorise the function(s) they need to. This offers much greater flexibility and cost-efficiency, ensuring that the system is built to the user needs.
Digital imaging ready – uncompromising ergonomics
A range of tubes are available for the BX3 range to suit every requirement. For the greatest system flexibility and user comfort, the tilting, trinocular U-TTR2 tube provides excellent eye piece height adjustment as well as interpupillary distance control, and the optical path slider can be attached to either side of the tube, once again this puts the user in full control of the microscope and ensures that the microscope adjusts to their posture and not vice versa.
Capturing everything correctly – Olympus digital camera range
It is always preferable to have a choice, and this is definitely true when it comes to matching your digital imaging requirements with your project work. For bright-field applications you’ll want dazzling colour fidelity and for fluorescence you’ll need the perfect monochrome capture. There are also those occasions where you would like a microscope camera that can do both. Add to this selection the ability to pick from a range of image sizes and resolutions, and the Olympus digital microscope camera range really does offer you the flexibility of choice.
Total system control – cellSens software for life science applications
Consisting of three different packages: cellSens Entry, cellSens Standard and cellSens Dimension, all user requirements can be met with ease. Incorporating a unique user-definable interface, the imaging process can be personalised from start to finish, for complete control over the entire experiment. Furthermore, each package integrates seamlessly with Microsoft Windows and Office programmes, making the advanced functionality of the Olympus cellSens family extremely user-friendly.
|Optical system||UIS2 optical system|
Coaxial coarse and fine focus with stage up and down mechanism
Focus stroke 25 mm
Coarse stroke 15 mm/rotation
Fine stroke 100 μm/rotation
Graduation on fine focus 1 μm
Prefocusing limit stopper and torque adjustment on coarse focus
Built-in Koehler illumination for transmitted light
Light intensity LED indicator, Built-in filters (LBD-IF, ND6, ND25) 12 V, 100 W halogen bulb (pre-centered)
Interchangeable reversed quintuple/sextuple/septuple nosepiece
Encoding optional with septuble nosepiece
Motorized septuple revolving nosepiece
Widefield tilting, telescopic and lifting binocular, inclined -3°-27°
Widefield tilting trinocular, inclined 5°–35°
Widefield trinocular, inclined 30°
Widefield erect image trinocular, inclined 30°
Widefield tilting binocular, inclined 5°–35°
Widefield ergo binocular, inclined 0°–25°
Widefield binocular, inclined 30°
Super widefield trinocular, inclined 24°
Super widefield erect image trinocular, inclined 24°
|Stage||Ceramic-coated coaxial stage with left or right hand low drive control, with rotating mechanism and torque adjustment mechanism, optional ergo grips available (Non stick coated grooved coaxial, plain, rotatable stages are also available)|
Swing out achromatic condenser (N.A. 0.9), for 1.25x–100x (swing-out: 1.25x–4x)
Achromatic aplanatic condenser (N.A. 1.4), for 10x–100x
Phase contrast, darkfield condenser (N.A. 1.1), [phase contrast: for 10x–100x, darkfield: for 10x–100x (up to N.A. 0.80)]
Universal condenser (N.A. 0.9), for 1.25x–100x [swing-out: 1.25x–4x,with oil top lens:(N.A. 1.4)]
Low magnification condenser (N.A. 0.75), for 2x–100x (Dry)
Ultra low magnification condenser (N.A. 0.16), for 1.25x–4x
Darkfield dry condenser (N.A. 0.8–0.92), for 10x–100x
Darkfield oil condenser (N.A. 1.20–1.40), for 10x–100x
Motorized universal condenser (N.A. 0.9, motorized 8-position turret, Aperture stop, polarizing filter in/out mechanism and top lens swing out mechanism), for 1.25x–100x [swing-out 1.25x-4x, with oil top lens: (N.A. 1.4)]
Manual reflected fluorescence, 8-position mirror turret unit, encoded with tool-free exchange of filter cubes
Motorized reflected fluorescence, 8-position mirror turret unit, encoded with tool-free exchange of filter cubes
|Fluorescence light source||
100 W Hg apo lamp housing and transformer
100 W Hg lamp housing and transformer
75 W Xe lamp housing and transformer
Fibre coupled metal-halide lightsources
Fibre coupled LED lightsource
|Controller||Optional: Control box for semi-motorised setups|
Ambient temperature: 5 ºC to 40 ºC (41 ºF to 104 ºF)
Maximum relative humidity: 80% for temperatures up to 31º C (88 ºF), decreasing linearly through 70% at 34 ºC (93 ºF), 60% at 37 ºC (99 ºF), to 50% relative humidity at 40 ºC (104 ºF)
Supply voltage fluctuations: Not to exceed ±10% of the normal voltage